Nitrate esters

ABSTRACT

Aliphatic Nitrate esters having a sulfur or phosphorus atom  beta  or  gamma  to a nitrate group having efficacy as vasodilators are described. Preferred nitrate esters may be synthesized by nitration of a 3-bromo-1,2-propanediol, and subsequent reaction to yield the desired mono, di or tetra nitrate ester.

FIELD OF INVENTION

This invention relates to novel aliphatic nitrate esters and use thereof as nitro vasodilators. More particularly this invention relates to nitrate esters bearing a sulfur or phosphorus atom β or γ to a nitrate group which have therapeutic utility as vasodilators and nitric oxide pro-drugs and/or substitutes for nitroglycerin in therapy.

BACKGROUND OF INVENTION

Glyceryl trinitrate (GTN) or nitroglycerin has been used as a vasodilator in the treatment of angina pectoris for over a hundred years, and is now believed to exert its therapeutic effect through in-vivo release of nitric oxide (NO), which itself has been identified as Endothelium Derived Relaxing Factor (EDRF). Other organic nitrates, such as isosorbide dinitrate, have also been identified as effective and clinically important vasodilators. Unfortunately, the use of GTN is not without its disadvantages, a principal of which is the tolerance effect which builds up quite rapidly over a relatively short period of time and markedly reduces the efficacy of GTN as a vasodilator.

A substantial body of evidence supports the hypothesis that the vasodilatory activity of organic nitrates is largely the result of activation of guanylate cyclase (Gcase) leading to vascular smooth muscle relaxation. GTN must undergo biotransformation in vivo and it is proposed that tolerance development may be associated with this need for biotransformation; the exact mechanism of which remains unresolved. Proposed sulfhydryl-dependent pathways include enzymic and non-enzymic biotransformation by a thiol. Indeed, the non-enzymic interaction of GTN with a limited range of thiols such as cysteine, N-acetylcysteine and thiosalicylic acid leads to activation of Gcase with an EC₅₀ in the submillimolar range in vitro. Regardless of the exact mechanism of biotransformation of GTN in vivo, it is postulated herein that if sulfur-containing functionalities are incorporated into the structure of nitrate esters, such molecules have the potential to activate Gcase and release •NO without reliance on GTN biotransformation pathways. Based upon bioassay data it is postulated that phosphorus-containing functionalities so incorporated might have similar potential. Thus, there is a need for synthetic aliphatic nitrate esters containing sulfur or phosphorus functionalities that may substitute for nitroglycerin as therapeutic agents.

OBJECT OF INVENTION

It is an object of the present invention to provide novel aliphatic nitrate esters bearing a sulfur or phosphorus atom β or γ to a nitrate group.

Another object of the present invention is to provide methods for making the novel S or P-containing nitrate esters.

Yet another object of the present invention is to provide novel pro-drugs for use as vasodilators.

BRIEF DESCRIPTION OF INVENTION

By one aspect of this invention there is provided aliphatic nitrate esters containing at least one nitrate group, in which a S or P atom is situated β or γ to a nitrate group, having the general formula; ##STR1## where X is CH₂ O, NMe, S, S₃ M, PO₃ HM, PO₃ M₂, SH, SR₇, P(O)(OR₅)(OR₆), P(0)(OR₅)(OM), P(O)(OR₅)(R₆), P(O)(OM)R₆, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR₈ or S(O)₂ OR₉ ;

Y is zero, SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, PO₃ HM, PO₃ M₂, P(O)(OR₅)(OM), or P(O)(OR₅)OR₆ ;

R₅, R₆, R₈, R₉, R₁₀ are the same or different alkyls containing 1-12 carbon atoms or C₁ or C₂ connections to R₁ -R₃ in cyclic derivatives;

R₇ is C₁ -C₈ alkyl or acyl;

R₂ and R₄ are the same or different and selected from H, ONO₂, C₁ -C₄ alkyl optionally bearing 1-3 nitrate groups, and acyl groups (--C(O)R₁₀);

R₁ and R₃ are the same or different and selected from H, C₁ -C₄ alkyl and chains, which may include one O, linking R₁ and R₃ to form pentosyl, hexosyl, cyclopentyl or cycohexyl rings, which rings optionally bear hydroxyl substituents; and

M is H, Na⁺, K⁺, NH₄ ⁺ or NH_(n) R₇(4-n), where n is 0-3.

By another aspect of this invention there is provided a pharmaceutical composition comprising an effective amount of an aliphatic nitrate ester having the formula ##STR2## where X is CH₂, O, NH, NMe, S, SO₃ M, PO₃ HM, PO₃ M₂, SH, SR₇, P(O)(OR₅)(OR₆), P(0)(OR₅)(OM), P(O)(OR₅)(R₆), P(O)(OM)R₆, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR₈ or S(O)₂ OR₉ ;

Y is zero, SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, PO₃ HM, PO₃ M₂, or P(O)(OR₅)(OM), P(O)(OR₅)OR₆ ;

R₅, R₆, R₈, R₉, R₁₀ are the same or different alkyls containing 1-12 carbon atoms or C₁ or C₂ connections to R₁ or R₃ in cyclic derivatives;

R₇ is C₁ -C₈ alkyl or acyl;

R₂ and R₄ are the same or different and selected from H, ONO₂, C₁ -C₄ alkyl optionally bearing 1-3 nitrate groups, and acyl groups (--C(O)R₁₀);

R₁ and R₃ are the same or different and selected from H, C₁ -C₄ alkyl and chains, which may include one O, linking R₁ and R₃ to form pentosyl, hexosyl, cyclopentyl or cycohexyl rings, which rings optionally bear hydroxyl substituents; and

M is H, Na⁺, K⁺, NH₄ ⁺ or Nh_(n) R₇(4-n),,where n is 0-3.

in admixture with a physiologically acceptable carrier therefore.

By yet another aspect of this invention there is provided a method for effecting tissue relaxation in a patient in need thereof comprising administering to said patient an effective amount of an aliphatic nitrate ester having the formula: ##STR3## where X is CH₂, O, NH, NMe, S, SO₃ M, PO₃ HM, PO₃ M₂, SH, SR₇, P(O)(OR₅)(OR₆), P(0)(OR₅)(OM), P(O)(OR₅)(R₆), P(O)(OM)R₆, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR₈ or S(O)₂ OR₉ ;

Y is zero, SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, PO₃ HM, PO₃ M₂, P(0)(OR₅)(OM), or P(O)(OR₅)OR₆ ;

R₅, R₆, R₈, R₉, R₁₀ are the same or different alkyls containing 1-12 carbon atoms or C₁ or C₂ connections to R₁ or R₃ in cyclic derivatives;

R₇ is C₁ -C₈ alkyl or acyl;

R₂ and R₄ are the same or different and selected from H, ONO₂, C₁ -C₄ alkyl optionally bearing 1-3 nitrate groups, and acyl groups (--C(O)R₁₀);

R₁ and R₃ are the same or different and selected from H, C₁ -C₄ alkyl and chains, which may include one O, linking R₁ and R₃ to form pentosyl, hexosyl, cyclopentyl or cycohexyl rings, which rings optionally bear hydroxyl substituents; and

M is H, Na⁺, K⁺, NH₄ ⁺ or NH_(n) R₇(4-n),, where n is 0-3.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

Simple nitrate esters according to this invention contain one or more nitrate groups in which a S or P atom is situated β or γ to a nitrate group. Accordingly, a general formula for the esters of this invention is: ##STR4## where X is CH₂, O, NH, NMe, S, SO₃ M, PO₃ HM, PO₃ M₂, SH, SR₇, P(O)(OR₅)(OR), P(0)(OR₅)(OM), P(O)(OR₅)(R₆), P(O)(OM)R₆, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR₈ or S(O)₂ OR;

Y is zero, SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, PO₃ HM, PO₃ M₂, P(O)(OR₅)(OM), or P(O)(OR₅)OR₆ ;

R₅, R₆, R₈, R₉, R₁₀ are the same or different alkyls containing 1-12 carbon atoms or C₁ or C₂ connections to R₁ or R₃ in cyclic derivatives;

R₇ is C₁ -C₈ alkyl or acyl;

R₂ and R₄ are the same or different and selected from H, ONO₂, C₁ -C₄ alkyl optionally bearing 1-3 nitrate groups, and acyl groups (--C(O)R₁₀);

R₁ and R₃ are the same or different and selected from H, C₁ -C₄ alkyl and chains, which may include one O, linking R₁ and R₃ to form pentosyl, hexosyl, cyclopentyl or cycohexyl rings, which rings optionally bear hydroxyl substituents; and

M is H, Na⁺, K⁺, NH₄ ⁺ or NH_(n) R₇(4-n),, where n is 0-3.

Compounds according to the present invention fall into five main categories having the formulae: ##STR5## where R is SO₃ M and M is Na⁺ or K⁺ ##STR6## The compound of formula 1 was synthesized from 3-bromo-1,2-propanediol by dropwise addition into a cold mixture of nitric acid (68-70%, 4.0 eq) and sulfuric acid (95%, 4.0 eq) in methylene chloride CH₂ Cl₂ (50 ml) and reaction at room temperature for 30 minutes. The organic layer was separated, washed, dried and concentrated to yield a yellow oil which was purified by silica gel flash chromatography to give a 45% yield of 3-bromo-1,2,propanediol dinitrate, ##STR7## was prepared by reacting compound 7 with an equimolar portion of Na₂ S₂ O₃ in 3:1 MeOH/H₂ O at 50° C. for 10 hours and subsequently purifying by silica gel flash chromatography.

The Bunte salt 2 was oxidized with a small molar excess of hydrogen peroxide H₂ O₂ (30%) in an ethanol: water mixture (1:1) with a catalytic amount of sulfuric acid for 2 days. Extraction with methylene chloride CH₂ Cl2, concentration, and purification by silica gel flash chromatography produced a 47% yield of the tetra-nitrate: ##STR8## The ¹³ C NMR spectra of 1 and 2 revealed 6 and 3 signals respectively, as expected from the presence of two chiral centres in 1 and only one in 2 (Table 1). The H NMR spectra of glycerol dinitrate derivatives (1, 2, 6) are of interest because of the large geminal coupling at the nitrated methylene and small or unobservable geminal coupling at the other methylene position (Table 1).

                                      TABLE 1                                      __________________________________________________________________________     NMR, IR, m.p. and mass spec characteristics of nitrates 1-4.sup.a              m.p.   .sup.1 H-NMR(ppm).sup.b                                                                           .sup.13 C-NMR(ppm).sup.b                                                                IR(cm.sup.-1)                                                                           MS(m/z, intensity                  __________________________________________________________________________                                                 %)                                 1 Liquid                                                                              5.43-5.55(2H, m), 4.84-8.93(2H, m), 4.60-                                                         77.08/77.00                                                                             (neat), 1634, 1270                                                                      (C1, C1.sup.-), 429(M+C1,                 4.69(2H, dd, J=6, 13Hz), 2.97-3.16(4H, m).                                                        69.33/69.29,                                                                            1042, 995, 855.                                                                         100), 393(M-1, 10).                                          37.05/36.89.                                         2 86° C.(dec.)                                                                 (DMSO-d.sub.6), 5.75-5.80(1H, m), 4.99-                                                           (DMSO-d.sub.6), 79.02                                                                   (KBr), 1638, 1449,                                                                      (ES.sup.+, Na), 323(M+Na,                 5.07(1H, dd, J=3.13Hz), 4.77-4.86(1H, dd,                                                         70.97, 32.04                                                                            1378, 1351, 1290,                                                                       53).                                      J=6.13Hz), 3.20-3.23(2H, d, J=7Hz)                                                                         1210, 1042, 654.                            3 65-66° C.                                                                    5.80-5.87(1H, m), 4.67-4.75(1H, dd, J=5,                                                          76.95, 69.88, 48.66                                                                     (KBr), 1651, 1344,                                                                      (C1, C1.sup.-), 2.18                      11Hz), 4.50-4.57(1H, dd, J=2, 11Hz),                                                                       1286, 1139, 937.                                                                        (M+C1, 100).                              3.69-3.80(1H, dd, J=8, 15Hz), 3.35-3.44                                        (1H, dd, J=3, 15Hz).                                                    4 64-65° C.                                                                    5.84-5.90(1H, m), 4.98-5.06(1H, dd,                                                               80.49, 75.07, 64.04                                                                     (KBr), 1649, 1339,                                                                      (C1, C1.sup.-), 202(M+C1,                 J=4, 12Hz), 4.77-4.83(1H, d, J=12Hz),                                                                      1287, 1122, 926.                                                                        100)                                      3.50-3.58(1H, dd , J=2, 15Hz), 3.31-3.42                                       (1H, dd, J=7, 15Hz).                                                    __________________________________________________________________________      .sup.a All compounds were characterized by elemental analysis or high          resolution mass spec., hplc and NMR analysis for homogeneity.                  .sup.b CDCl.sub.3 was used as solvent for .sup.1 H--, .sup.13 CNMR, unles      otherwise indicated.                                                     

Nitration of bis-(2,3-dihydroxypropyl) disulfide ##STR9## in the biphasic CH₂ Cl₂ /aqueous HNO₃ H₂ SO₄ medium described above yielded, after chromatographic silica gel purification 5% and 10% yields of the sultone 3 and sultine 4, respectively. The anticipated tetra nitrate 1 was not obtained. NMR spectra obtained for these products were highly solvent-dependent and were similar to those of the glycerol dinitrates, but with the significant difference that the large geminal coupling is associated with the upfield rather than the downfield methylene protons (Table 1). Definitive structure identification rested upon mass spectral data: soft chemical ionization with C1 ion capture determined 3 and 4 to be the sultone and sultine, respectively.

The compound of formula 5 was synthesized by two methods. In the first method, 1-bromo-2,3-propanediol, 6, was silylated to provide a substrate for the Arbuzov reaction with triethyl phosphite. The resulting phosphonate was nitrated using nitronium tetrafluoroborate to yield the product 5, which was isolated after silica flash chromatography. In method 2, an Arbuzov reaction with 1-bromo-2,3-epoxypropane yields the 1-phosphono-2,3-epoxypropane, which is converted to the dinitrate 5 using the same procedure detailed for synthesis of 7. Isolation after chromatography yields the product 5 with: ³¹ P NMR δ=23 ppm; ¹³ C NMR δ=16, 27, 62, 71, 75 ppm; ¹ H NMR δ=1.3, 2.2, 4.1, 4.4-5.0, 5.5 ppm.

Activation of soluble Gcase by nitrates 1-4 was assayed, employing partially purified enzyme freshly prepared from rat aorta homogenates, using the radio immunoassay method described by Bennett et al, Can. J. Physiol. Pharmacol. 1992, 70, 1297, the disclosure of which is incorporated herein by reference.

Dose-response curves were obtained for Gcase activation by nitrates 1-4 and GTN in the presence and absence of cysteine and dithiothretol (DTT; both 2 mM). The data from these curves are summarized in Table 2, which gives: (a) concentrations of nitrates required to give a response equivalent to the maximal response seen for GTN+cysteine, and (b); the maximal response measured for each nitrate. The Gcase assay data shows that dinitrate 2 activates Gcase, with a submillimolar EC₅₀, in the absence of any added thiol, in contrast to GTN which requires added cysteine. Compounds 2 and 4 also activate Gcase in the presence of DTT in contrast to GTN. The activity of the tetra nitrate 1 is very low and entirely equivalent to glycerol-1,2-dinitrate in this assay. No activation of Gcase by glycerol mononitrates is observed in this assay.

                                      TABLE 2                                      __________________________________________________________________________     Summary of guanylyl cyclase activation dose response curves for nitrates       2-4 in presence and                                                            absence of thiols (2 mM) relative to GTN + 2 mM cysteine.                                GTN + cys                                                                            2 no thiol                                                                          2 + cys.                                                                           2 + DTT                                                                             3 + cys.                                                                            4 + cys.                                                                           4 + DTT                                 __________________________________________________________________________     % maximal response.sup.b                                                                 100(5)                                                                               105(12)                                                                             293(15)                                                                            164(23)                                                                             1087(200)                                                                           98(16)                                                                             174(30)                                 concentration at max.                                                                    1.0   0.5  5.0 5.0  1.0  5.0 1.5                                     response (mM).sup.c                                                            GTN equivalence                                                                          1.0   0.4  0.7 2.0  0.2  5.0 0.8                                     concentration(mM).sup.d                                                        __________________________________________________________________________      .sup.a Average of 4-6 experiments using 2-3 separate preparations. Variou      nitrates gave no response above basal concentrations: GTN, GTN + DTT, 1,       3, 3 + DTT, 4, glycerol1,2-dinitrate + cysteine. Response to 1 (+DTT or        +cys.) was discernible but <20%. Several responses do not plateau, thus        EC.sub.50 values cannot be quoted.                                             .sup.b Maximal response to GTN + cys. Ranged from 360-540 pmol/min.mg in       separate experiments and was set at 100%. Other responses relative to          maximal GTN + cys. response are expressed as percentages with standard         errors.                                                                        .sup.c Nitrate concentration at which maximal response is observed:            concentrations did not exceed 5 mM (1 mM for 3).                               .sup.d Nitrate concentration at which response reaches maximal response t      GTN + cysteine (ie. 100%).                                               

In order to extend the Gcase data, the relaxing effects of nitrates 2,3 and 4 on rat aortic tissue were examined. Thoracic aortic strips were prepared from male Sprague-Dawley rats (Charles-River, Canada) as described in McGuire et al, J. Exp. Ther. 1994, 271,708 and Stewart et al, Can. J. Physiol Pharmacol 1989, 67, 403, incorporated herein by reference. Tissues were contracted submaximally with phenylephrine (0.1 μM) and exposed to various concentrations of nitro vasodilator to obtain concentration-response curves. Compared to the control experiments, in this intact tissue assay, all three nitrates were observed to cause significant tissue relaxation. The EC₅₀ values for 2,3 and 4 were 3.94 μM, 3.37 μM and 9.06 μM respectively. In similar rat aorta relaxation assays, a nitrosothiol (BU^(t) SNO) and GTN itself were seen to give EC₅₀ values of 5 μM and 8.3 nM, respectively.

Three of the four sulfur-containing nitrates described herein, two mononitrates (3,4) and one dinitrate (2), are shown to activate Gcase at a higher maximal even than GTN. In addition, compound 2 does not require added thiol for activation. The significant relaxing effects of 2,3 and 4 on rat aortic tissue are compatible with Gcase activation data. The positioning of a sulfur atom β or γ to the nitrate group may allow intramolecular interaction of S with the nitrate N via a five-membered ring. 

We claim:
 1. Aliphatic nitrate esters containing at least one nitrate group, in which a S or P atom is situated β or γ to a nitrate group, having the general formula: ##STR10## where X is CH₂, O, NH, NMe, S, SO₃ M, PO₃ HM, PO₃ M₂, SH, SR₇, P(O)(OR₆)(OR₆), P(O)(OR₆)(OM), P(O)(OR₅)(R₆), P(O)(OM)R₆, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR₈ or S(O)₂ OR₉ ;Y is SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, PO₃ HM, PO₃ M₂, P(O)(OR₅)(OM), or P(O)(OR₅)(OR₆), or does not exist; R₅, R₆, R₈, R₉, R₁₀, are the same or different alkyls containing 1-12 carbon atoms or C₁ or C₂ connections to R₁ or R₃ in cyclic derivatives; R₇ is C₁ -C₈, alkyl or acyl; R₂ and R₄ are the same or different and selected from H, ONO₂, C₁ -C₄ alkyl optionally bearing 1-3 nitrate groups, and acyl groups (--C(O)R₁₀); R₁ and R₃ are the same or different and selected from H, C₁ -C₄ alkyl and chains, which may include one O, linking R₁ and R₃ to form pentosyl, hexosyl, cyclopentyl or cycohexyl rings, which rings optionally bear hydroxyl substituents; and M is H, Na⁺, K⁺, NH₄ ⁺ or N⁺ H_(n) R₁₁(4-n), where n is 0-3; and with the proviso that, when R₆ is an alkyl group, and R, R₂ and R₄ ═H, R₃ is not H or methyl.
 2. Aliphatic nitrate esters as claimed in claim 1 wherein:X is selected from the group consisting of CH₂, O, NH, NMe, S, SO₃ M, SO, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR₈ and S(O)₂ OR₉ ; and Y is selected from the group consisting of zero, SR₄, SR₁₀, SSR₁₀, SO₂ M and SO₃ M.
 3. Aliphatic nitrate esters as claimed in claim 2, wherein Y does not exist.
 4. Aliphatic nitrate esters as claimed in claim 1, having a formula selected from the group consisting of: ##STR11## where M is Na⁺ or K⁺ ##STR12##
 5. A pharmaceutical composition comprising an effective amount of an aliphatic nitrate ester having the formula: ##STR13## where X is CH₂, O, NH, NMe, S, SO₃ M, PO₃ HM, PO₃ M₂, SH, SR₇, P(O)(OR₅)(OR₆), P(O)(OR₆)(OM), P(O)(OR₆)(R₆), P(O)(OM)R₆, SO₂ M, S(O)R₆, S(O)₂ R₉, S(O)OR₈ or S(O)₂ OR₉ ;Y is SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, PO₃ HM, PO₃ M₂, P(0)(OR₅)(OM), or P(O)(OR₅)(OR₆), or does not exist; R₅, R₆, R₈. R₉, R₁₀, are the same or different alkyls containing 1-12 carbon atoms or C₁ or C₂ connections to R₁ or R₃ in cyclic derivatives; R₇ is C₁ -C₈, alkyl or acyl; R₂ and R₄ are the same or different and selected from H, ONO₂, C₁ -C₄ alkyl optionally bearing 1-3 nitrate groups, and acyl groups (--C(O)R₁₀); R₁ and R₃ are the same or different and selected from H, C₁ -C₄ alkyl and chains, which may include one O, linking R₁ and R₃ to form pentosyl, hexosyl, cyclopentyl or cycohexyl rings, which rings optionally bear hydroxyl substituents; and M is H, Na⁺, K⁺, NH₄ ⁺ or N⁺ H_(n) R₁₁(4-n, where n is 0-3; and with the proviso that, when R₆ is an alkyl group, and R₁, R₂ and R₄ ═H, R₃ is not H or methyl; in admixture with a physiologically acceptable carrier therefor.
 6. A pharmaceutical composition as claimed in claim 5, whereinX is selected from the group consisting CH₂, O, NH, NMe, S, SO₃ M, SO, SO₂ M, S(O)R₈, S(O)₂ R₉, S(O)OR, or S(O)₂ OR₉ ; and Y is selected from the group consisting of SR₄, SR₁₀, SSR₁₀, SO₂ M, SO₃ M, or does not exist.
 7. A pharmaceutical composition as claimed in claim 5, wherein said nitrate ester has a formula selected from the group consisting of ##STR14## where M is Na⁺ or K⁺ ##STR15## 